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检测<em>原位</em>蛋白质 - 蛋白质复合物的<em>果蝇</em>幼虫神经肌肉接头使用接近结扎分析

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Neurociência

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JoVE Journal Neurociência

Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

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检测<em>原位</em>蛋白质 - 蛋白质复合物的<em>果蝇</em>幼虫神经肌肉接头使用接近结扎分析

DOI:

10.3791/52139-v

•

10:31 min

•

January 20, 2015

•

Simon Wang, SooHyun Yoo, Hae-yoon Kim, Mannan Wang, Clare Zheng, Wade Parkhouse, Charles Krieger, Nicholas Harden

¹Department of Molecular Biology and Biochemistry,Simon Fraser University, ²Department of Biomedical Physiology and Kinesiology,Simon Fraser University

Capítulos

00:05Título
02:09Dissection
04:25Fixation, Permeabilization, and Immunochemistry
06:12Proximity Ligation Assay and Mounting
08:06Results: Detection of Endogenous Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction
09:51Conclusion

Summary

Tadução automática

此协议演示如何接近结扎检测试剂盒可用于检测原位蛋白质-蛋白质相互作用的果蝇幼虫神经肌肉接头。利用这种技术，光盘大和胡立泰邵示，以形成一复合的突触后区域，预先通过共免疫沉淀鉴定的关联。

Tags

Protein-protein Complex Discs Large (Dlg)Hu-li Tai Shao (Hts)Neuromuscular Junction Drosophila Larva Proximity Ligation Assay In Situ Detection Postsynaptic Region Scaffolding Protein Adducin Homologue Co-immunoprecipitation

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