Journal
/
神经科学
/
Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

DOI:
10.3791/52139-v

•

10:31 min

•

January 20, 2015

•

, , , , , , ,
1Department of Molecular Biology and Biochemistry,Simon Fraser University, 2Department of Biomedical Physiology and Kinesiology,Simon Fraser University

Chapters

  • 00:05Title
  • 02:09Dissection
  • 04:25Fixation, Permeabilization, and Immunochemistry
  • 06:12Proximity Ligation Assay and Mounting
  • 08:06Results: Detection of Endogenous Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction
  • 09:51Conclusion

Summary

自动翻译

自动翻译

This protocol demonstrates how Proximity Ligation Assay can be used to detect in situ protein-protein interactions at the Drosophila larval neuromuscular junction. With this technique, Discs large and Hu-li tai shao are shown to form a complex at the postsynaptic region, an association previously identified through co-immunoprecipitation.

Tags

Protein-protein ComplexDiscs Large (Dlg)Hu-li Tai Shao (Hts)Neuromuscular JunctionDrosophila LarvaProximity Ligation AssayIn Situ DetectionPostsynaptic RegionScaffolding ProteinAdducin HomologueCo-immunoprecipitation

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/52139?article_type=v

Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
check_url/cn/52139?article_type=v

In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
check_url/cn/52139?article_type=v

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction
check_url/cn/52139?article_type=v

Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction
check_url/cn/52139?article_type=v

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
check_url/cn/52139?article_type=v

Why Quantification Matters: Characterization of Phenotypes at the Drosophila Larval Neuromuscular Junction
check_url/cn/52139?article_type=v

Measuring Neuromuscular Junction Functionality
check_url/cn/52139?article_type=v

Two Algorithms for High-throughput and Multi-parametric Quantification of Drosophila Neuromuscular Junction Morphology
check_url/cn/52139?article_type=v

Focal Macropatch Recordings of Synaptic Currents from the Drosophila Larval Neuromuscular Junction
check_url/cn/52139?article_type=v

Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

Read Article