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Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

11,665 Views

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10:02 min

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December 12, 2014

DOI:

10.3791/52100-v

DOI:
10.3791/52100-v

•

10:02 min
December 12, 2014

•

3 Views
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1INSERM, U968, 2Sorbonne Universités, UPMC Paris 06, UMR_S 968,Institut de la Vision, 3CNRS, UMR_7210, 4Department of Biological Sciences, Center for Cancer, Genetic Diseases and Gene Regulation,Fordham University

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Photoreceptor Outer SegmentsRetinal Pigment Epithelial CellsPhagocytosis AssayPorcineBovineSucrose GradientPhotopigmentFITC LabelingFluorescence QuantificationOxidative StressAging

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Parinot, C., Rieu, Q., Chatagnon, J., Finnemann, S. C., Nandrot, E. F. Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells. J. Vis. Exp. (94), e52100, doi:10.3791/52100 (2014).

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