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JoVE Journal Neuroscience
Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
 

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Article DOI: 10.3791/52029-v • 11:09 min • October 30th, 2014
October 30th, 2014 •
1, *1, *2,3, 2,4, 1
1Department of Molecular and Experimental Medicine, Dorris Neuroscience Center, The Scripps Research Institute, 2Department of Cellular and Molecular Medicine, University of California San Diego, 3Department of Bioengineering, University of California San Diego, 4Department of Neurosciences, University of California San Diego School of Medicine
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.

Tags

Cargo Mapping Analysis Molecular Motors Axonal Cargo Quantitative Fluorescence Microscopy Motor/cargo Associations Live Imaging Microfluidic Devices Chemical Fixation Immunofluorescence Staining Colocalization Sub-pixel Position Coordinates Gaussian Functions Point Spread Functions Fixed Cargo And Motor Images Tracked Trajectories
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