Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Paired Whole Cell Recordings in Organotypic…
JoVE Journal
Neuroscience

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Neuroscience
Paired Whole Cell Recordings in Organotypic Hippocampal Slices
 

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Article DOI: 10.3791/51958-v • 09:23 min • September 28th, 2014
September 28th, 2014 •
1, 2, *2, *1
1Department of Physiology and Centre for Brain Research, University of Auckland, 2Department of Molecular and Cellular Physiology, Stanford University
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Pair recordings are simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling precise electrophysiological and pharmacological characterization of the synapses between individual neurons. Here we describe the detailed methodology and requirements for establishing this technique in organotypic hippocampal slice cultures in any laboratory equipped for electrophysiology.

Tags

Paired Whole Cell Recordings Organotypic Hippocampal Slices Synaptically Connected Neurons Electrophysiological Characterization Synaptic Connections Pharmacological Manipulation Presynaptic Neuron Postsynaptic Neuron Organotypic Hippocampal Slice Cultures Identification Of Cell Types Synaptic Function Native Brain Tissue Properties Of Synaptic Transmission Plasticity Extracellular Axonal Stimulation Challenging Technique Whole Cell Patch Clamping Hardware And Methodological Criteria Attaining Synaptically Connected Paired Recordings CA3
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter