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Immunology and Infection

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JoVE Journal Immunology and Infection
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
 

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Article DOI: 10.3791/51894-v • 06:40 min • September 9th, 2014
September 9th, 2014 •
1, 1, 1, 1
1Department of Pathology, Northwestern University

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Resumo
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We present an in vitro mouse fetal liver erythroblast culture system that dissects the early and late stages of terminal erythropoiesis. This system facilitates functional analysis of specific genes in different developmental stages.

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Mouse Fetal Liver Culture System Target Gene Functions Early Stages Late Stages Terminal Erythropoiesis Erythroid Burst Forming Units (BFU-Es) Erythroid Colony Forming Units (CFU-Es) Gene Functions Chronological Manner Purified Erythroblasts Exogenous Expression CDNAs Small Hairpin RNAs (shRNAs) Growth Factors Erythropoietin (Epo) Progenitor Stage Cell Differentiation Cell Proliferation
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