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Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells

Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells

DOI:
10.3791/51859-v

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08:12 min

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August 11, 2014

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, , ,
1Department of Pathology, Institute of Cell Engineering,Johns Hopkins University, 2Department of Internal Medicine I,University of Regensburg, 3Department of Rheumatology,Asklepios Medical Center

Capítulos

  • 00:05Título
  • 01:32Generation of HLA-A2-Ig Based Killer Artificial Antigen Presenting Cells (KaAPC)
  • 03:19Quality Control, Peptide Loading, Washing, Storage, and Stability of KaAPC
  • 04:53KaAPC In Vitro Killing Assay
  • 06:23Results: KaAPC Eliminate CTL in an Antigen-specific Fashion
  • 07:43Conclusion

Summary

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Guidelines are presented for the generation of killer artificial antigen presenting cells, KaAPC, an efficient tool for in vitro depletion of human antigen-specific T cells and an alternative solution to cellular immunotherapy for the treatment of T cell-mediated autoimmune diseases in an antigen-specific fashion without compromising the remaining immune system.

Tags

Keywords: Killer Artificial Antigen Presenting CellsKaAPCIn Vitro T Cell DepletionAutoimmune Disease TreatmentCell-based ImmunotherapyAntigen-specific T Cell ModulationGlobal ImmunosuppressionTolerance-inducing Antigen Presenting Cells

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