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השתפר בג'ל Sulfo-glycomics β-ביעור עבור מקיף O צמוד וReductive ידי ספקטרומטריית מסה

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Química

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JoVE Journal Química

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

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השתפר בג'ל Sulfo-glycomics β-ביעור עבור מקיף O צמוד וReductive ידי ספקטרומטריית מסה

11,649 Views

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13:06 min

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November 20, 2014

DOI:

10.3791/51840-v

DOI:

10.3791/51840-v

•

13:06 min

November 20, 2014

•

4 Views

David B. Nix², Tadahiro Kumagai, Toshihiko Katoh³, Michael Tiemeyer², Kazuhiro Aoki

¹Complex Carbohydrate Research Center,University of Georgia, ²Department of Biochemistry and Molecular Biology,University of Georgia, ³Research Institute for Bioresources and Biotechnology,Ishikawa Prefectural University

Tags

In-gel Reductive β-elimination O-linked Glycans Sulfo-glycans SDS-PAGE Mass Spectrometry Glycomic Analysis Sulfoglycomic Analysis Glycoproteins Permethylation Phase-partition

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