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Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
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JoVE Journal Chemie
Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

11,660 Views

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13:06 min

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November 20, 2014

DOI:

10.3791/51840-v

DOI:
10.3791/51840-v

•

13:06 min
November 20, 2014

•

2 Views
, , , ,
1Complex Carbohydrate Research Center,University of Georgia, 2Department of Biochemistry and Molecular Biology,University of Georgia, 3Research Institute for Bioresources and Biotechnology,Ishikawa Prefectural University

Summary

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In order to comprehensively explore the diversity of O-linked glycans, a new procedure for in-gel reductive β-elimination, combined with permethylation and a rapid phase-partition method, is applied to the analysis of O-linked glycans directly released from glycoproteins resolved by SDS-PAGE and amenable to subsequent glycomic analysis by mass spectrometry.

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In-gel Reductive β-eliminationO-linked GlycansSulfo-glycansSDS-PAGEMass SpectrometryGlycomic AnalysisSulfoglycomic AnalysisGlycoproteinsPermethylationPhase-partition

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Cite this Article

Nix, D. B., Kumagai, T., Katoh, T., Tiemeyer, M., Aoki, K. Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry. J. Vis. Exp. (93), e51840, doi:10.3791/51840 (2014).

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