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ניתוח metabolomic של מוח חולדה על ידי ספקטרוסקופיה בתהודה מגנטית הגרעינית ברזולוציה גבוהה של תמציות רקמות
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Capítulos
- 00:05Título
- 01:13Extraction of Metabolites
- 02:40Phase Separation and Solvent Evaporation
- 04:41Preparation of NMR Samples and Performance of the 31P NMR Experiment for Brain Phospholipid Analysis
- 06:58Results: NMR Spectroscopic Analysis of Rat Brain Tissue
- 08:35Conclusion
The neurochemistry of mammalian brain is changed in many neurological and systemic diseases. Characteristic profiles of cerebral metabolites can be efficiently obtained based on crude extracts of brain tissue. To this end, high-resolution NMR spectroscopy is employed, enabling detailed quantitative analysis of metabolite concentrations (metabolomics).
