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Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
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神经科学
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JoVE 杂志 神经科学
Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

DOI:
10.3791/51829-v

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09:01 min

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September 21, 2014

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, ,
1Centre de Résonance Magnétique Biologique et Médicale, UMR 7339 CNRS, Faculté de Médecine,Aix-Marseille Université, 2Centre de Recherches en Oncologie Biologique et Oncopharmacologie, UMR 911 INSERM, Faculté de Médecine,Aix-Marseille Université

Chapters

  • 00:05Title
  • 01:13Extraction of Metabolites
  • 02:40Phase Separation and Solvent Evaporation
  • 04:41Preparation of NMR Samples and Performance of the 31P NMR Experiment for Brain Phospholipid Analysis
  • 06:58Results: NMR Spectroscopic Analysis of Rat Brain Tissue
  • 08:35Conclusion

Summary

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The neurochemistry of mammalian brain is changed in many neurological and systemic diseases. Characteristic profiles of cerebral metabolites can be efficiently obtained based on crude extracts of brain tissue. To this end, high-resolution NMR spectroscopy is employed, enabling detailed quantitative analysis of metabolite concentrations (metabolomics).

Tags

MetabolomicsNMR SpectroscopyRat BrainMetabolite QuantificationGlobal Metabolite ProfilingUnbiased Metabolite AnalysisPhenotype Characterization

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