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Dérivation sans chargeur-de cellules progénitrices de la crête neurale souches pluripotentes humaines cellules
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Neurociência
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JoVE Journal Neurociência
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Dérivation sans chargeur-de cellules progénitrices de la crête neurale souches pluripotentes humaines cellules

13,945 Views

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10:33 min

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May 22, 2014

DOI:

10.3791/51609-v

DOI:
10.3791/51609-v

•

10:33 min
May 22, 2014

•

6 Views
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1Developmental Biology, Center for Stem Cell Biology,Sloan-Kettering Institute for Cancer Research, 2St. Giles Laboratory of Human Genetics of Infectious Diseases,The Rockefeller University

Summary

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Crête neurale (NC) des cellules dérivées de cellules souches pluripotentes humaines (HPSC) ont un grand potentiel pour la modélisation du développement humain et de la maladie et des thérapies de remplacement cellulaire. Ici, une adaptation libre-alimentation de l'a largement utilisé<em> In vitro</em> Protocole de différenciation pour la dérivation de cellules NC de hPSCs est présenté.

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Cite this Article

Zeltner, N., Lafaille, F. G., Fattahi, F., Studer, L. Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells. J. Vis. Exp. (87), e51609, doi:10.3791/51609 (2014).

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