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Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

DOI:
10.3791/51522-v

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10:48 min

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May 15, 2014

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, , ,
1Department of Microbiology,Boston University School of Medicine

Capítulos

  • 00:05Título
  • 01:29Multi-stage Reporter Viruses
  • 02:27Plate Cells
  • 05:08Fixing Cells
  • 06:13Quantify Virus Growth
  • 07:29Alternative Protocol: Kinetic Plate Assays
  • 08:29Effect of Poxvirus Inhibitors on TrpV Expression
  • 10:10Conclusion

Summary

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We describe the usage of a fluorescent reporter vaccinia virus that enables real-time measurement of viral infectivity and gene expression through the stage-specific expression of spectrally distinct reporter fluorophores. We detail a plate-based method for accurately identifying the stage at which virus replication is affected in response to small molecule inhibition.

Tags

PoxvirusVaccinia VirusReporter VirusGene ExpressionFluorescent ProteinViral ReplicationHigh-throughputAntiviral DiscoveryVirus-host InteractionEvolutionary Biology

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