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Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
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Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
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May 15, 2014
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Summary
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We describe the usage of a fluorescent reporter vaccinia virus that enables real-time measurement of viral infectivity and gene expression through the stage-specific expression of spectrally distinct reporter fluorophores. We detail a plate-based method for accurately identifying the stage at which virus replication is affected in response to small molecule inhibition.
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Rozelle, D. K., Filone, C. M., Dower, K., Connor, J. H. Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression. J. Vis. Exp. (87), e51522, doi:10.3791/51522 (2014).
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