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Komplet Rygmarvsskader og Brain Dissektion Protokol for Efterfølgende whole- mount<em> In Situ</em> Hybridisering i Larver havlampret
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Neurociência
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JoVE Journal Neurociência
Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey

Komplet Rygmarvsskader og Brain Dissektion Protokol for Efterfølgende whole- mount<em> In Situ</em> Hybridisering i Larver havlampret

DOI:
10.3791/51494-v

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08:11 min

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October 14, 2014

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, , ,
1Centre for Neuroregeneration, School of Biomedical Sciences,University of Edinburgh, 2Shriners Hospitals Pediatric Research Center (Center for Neural Repair and Rehabilitation),Temple University School of Medicine, 3Department of Neurology,Temple University School of Medicine

Capítulos

  • 00:05Título
  • 01:22Preparing the Larval Sea Lamprey
  • 02:17Complete Spinal Cord Transection
  • 04:30Brain Dissection
  • 06:55Results: Expression of neogenin After Spinal Cord Transection
  • 07:35Conclusion

Summary

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Lampretter genvinde bevægelse efter en komplet rygmarvsskade. Men nogle spinale ragende neuroner er gode regeneratorer og andre er ikke. Dette papir illustrerer de teknikker til boliger havlampret larver (og for nylig transformerede voksne), der producerer komplette rygmarv transections og forberede whole- mount hjerner og rygmarv til in situ hybridisering.

Tags

Keywords: Spinal Cord InjuryRegenerationWholemount In Situ HybridizationLarval Sea LampreyBrainstem NeuronsPropriospinal AxonsAxon RegenerationNeuron-intrinsic MechanismsGene ExpressionComplete TransectionHousingAnesthesiaDissection Protocol

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