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Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons
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Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons
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February 25, 2014
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To understand the structure of neuronal networks, functional and morphological characterization of individual neurons is a necessity. Here, we demonstrate juxtasomal biocytin labeling, which allows electrophysiological recordings in the extracellular configuration, yet maintaining the ability to intracellularly label the neuron for post hoc reconstruction of dendritic and axonal architecture.
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Narayanan, R. T., Mohan, H., Broersen, R., de Haan, R., Pieneman, A. W., de Kock, C. P. Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons. J. Vis. Exp. (84), e51359, doi:10.3791/51359 (2014).
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