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Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
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Neurociência
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JoVE Journal Neurociência
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

15,592 Views

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14:11 min

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May 04, 2014

DOI:

10.3791/51276-v

DOI:
10.3791/51276-v

•

14:11 min
May 04, 2014

•

3 Views
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1Center for Neuroscience, Swammerdam Institute for Life Sciences,University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Section Molecular Cytology, Swammerdam Institute for Life Sciences,University of Amsterdam

Tags

Dendritic SpinesRat Primary Hippocampal NeuronsStructured Illumination Microscopy (SIM)Super-resolution MicroscopyNeuronal ConnectivitySynaptic PlasticityNeuron CultureFluorescent Protein3D Reconstruction

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Schouten, M., De Luca, G. M. R., Alatriste González, D. K., de Jong, B. E., Timmermans, W., Xiong, H., Krugers, H., Manders, E. M. M., Fitzsimons, C. P. Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy. J. Vis. Exp. (87), e51276, doi:10.3791/51276 (2014).

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