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En mus, to kulturer: Isolation and Culture of Adult nevrale stamceller fra de to nevrogene Soner Individuell Mus
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
DOI:

09:52 min

February 25, 2014

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Capítulos

  • 00:05Título
  • 01:41Microdissection of Mouse Brain
  • 04:13SVZ Tissue Dissociation
  • 05:43DG Tissue Dissociation
  • 07:36Cell Plating for Monolayer and Neurosphere Culture Generation
  • 08:17Results: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
  • 09:21Conclusion

Summary

Tadução automática

Her beskriver vi en detaljert protokoll for samtidig generering av nevrale forløper cellekulturer, som enten heft monolayers eller neurospheres, fra subventricular sone og dentate gyrus av individuelle voksne mus.

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