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一个鼠标，两种文化：隔离和成人神经的培养干细胞从单个小鼠的两个神经源性区

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

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一个鼠标，两种文化：隔离和成人神经的培养干细胞从单个小鼠的两个神经源性区

DOI:

10.3791/51225-v

•

09:52 min

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February 25, 2014

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Tara L. Walker, Gerd Kempermann²

¹Center for Regenerative Therapies Dresden,Technische Universität Dresden, ²German Center for Neurodegenerative Diseases (DZNE) Dresden

Capítulos

00:05Título
01:41Microdissection of Mouse Brain
04:13SVZ Tissue Dissociation
05:43DG Tissue Dissociation
07:36Cell Plating for Monolayer and Neurosphere Culture Generation
08:17Results: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
09:21Conclusion

Summary

Tadução automática

在这里，我们描述的同时产生神经前体细胞培养的一个详细的协议，因为无论是贴壁单层或神经球，从脑室下区和个体成年小鼠海马齿状回。

Tags

Keywords: Neural Stem Cells Neurosphere Assay Adherent Monolayer Culture Adult Neurogenesis Subventricular Zone Dentate Gyrus Cell Proliferation Cell Differentiation Mouse Models

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