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隔离和肉毒杆菌神经毒素定量从复杂的基质使用BoTest矩阵测定

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays

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隔离和肉毒杆菌神经毒素定量从复杂的基质使用BoTest矩阵测定

DOI:

10.3791/51170-v

•

12:25 min

•

March 03, 2014

•

F. Mark Dunning, Timothy M. Piazza, Füsûn N. Zeytin, Ward C. Tucker

¹BioSentinel Inc., Madison, WI

Capítulos

00:05Título
01:40Preparation of the Standard Curve
03:33Prepare Solid Food Unknown Samples
04:56Plate Setup and BoNT/A Pull Down
06:11Assay Initiation, Incubation, and Data Collection
07:14Results: BoTest Matrix Assay
11:43Conclusion

Summary

Tadução automática

该BoTest矩阵A型肉毒毒素（肉毒毒素）检测方法快速净化，并从各种样品基质的量化肉毒毒素。在这里，我们提出了一个协议，用于肉毒毒素，从固体和液体基质的检测和定量，并证明有肉毒杆菌，番茄，牛奶的检测。

Tags

Keywords: Botulinum Neurotoxin BoNT BoTest Matrix Assays Immunoprecipitation Fluorogenic Reporter Complex Matrices Pharmaceutical Testing Food Safety Rapid Detection Quantification Mouse Bioassay In Vitro Replacement Assays

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