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JoVE Journal Neuroscience
Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays
 

Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays

Article DOI: 10.3791/51170-v • 12:25 min • March 3rd, 2014
March 3rd, 2014 •
1, 1, 1, 1
1BioSentinel Inc., Madison, WI

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The BoTest Matrix botulinum neurotoxin (BoNT) detection assays rapidly purify and quantify BoNT from a range of sample matrices. Here, we present a protocol for the detection and quantification of BoNT from both solid and liquid matrices and demonstrate the assay with BOTOX, tomatoes, and milk.

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Keywords: Botulinum Neurotoxin BoNT BoTest Matrix Assays Immunoprecipitation Fluorogenic Reporter Complex Matrices Pharmaceutical Testing Food Safety Rapid Detection Quantification Mouse Bioassay In Vitro Replacement Assays
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