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Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
JoVE Journal
Biologia
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JoVE Journal Biologia
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

18,406 Views

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07:28 min

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February 27, 2014

DOI:

10.3791/51119-v

DOI:
10.3791/51119-v

•

07:28 min
February 27, 2014

•

15 Views
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1Huisken Lab,Max Planck Institute of Molecular Cell Biology and Genetics

Summary

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The development of zebrafish can be followed over days with light sheet microscopy when embryos are embedded in optically clear polymer tubes with low-concentration agarose.

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Light Sheet MicroscopyZebrafishEmbryonic DevelopmentPhoto-toxicityBleachingLong-term Time-lapse ImagingSample MountingAgaroseOptically Clear TubesReal-time Developmental Biology

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Cite this Article

Weber, M., Mickoleit, M., Huisken, J. Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish. J. Vis. Exp. (84), e51119, doi:10.3791/51119 (2014).

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