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设计一个连续和操作<sup> 13</sup> C和<sup> 15</sup>Ñ贴标商会统一或差分,代谢和结构,植物同位素标记
JoVE Journal
Ambiente
This content is Free Access.
JoVE Journal Ambiente
Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
DOI:

10:16 min

January 16, 2014

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Capítulos

  • 00:05Título
  • 01:26Chamber Construction
  • 02:37Temperature, Humidity, and CO2 Controls
  • 04:51Irrigation System and Potting Plants
  • 06:23Fertilization with a 15N-labeled Fertilizer
  • 07:22Harvest
  • 08:14Results: Successful Incorporation of 13C and 15N into Plant Tissue
  • 09:51Conclusion

Summary

Tadução automática

这个方法介绍了如何建立和运营一个连续13 C和15 N同位素标记室统一或差分植物组织标签。从代谢和须芒草gerardii结构标记代表结果进行了讨论。

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