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优化高等级胶质瘤细胞培养从外科标本用于临床相关的动物模型和3D免疫化学
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry

优化高等级胶质瘤细胞培养从外科标本用于临床相关的动物模型和3D免疫化学

DOI:

12:25 min

January 07, 2014

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Capítulos

  • 00:05Título
  • 02:38Single Cell GBM (Glioblastoma) Suspension for Neurosphere Culture
  • 05:13Morphological and Molecular Analysis of Neurospheres by Immunohistochemistry
  • 08:35Results: Representative Xenograft Tumor Formation from High Grade Glioma Cell Cultures
  • 11:12Conclusion

Summary

Tadução automática

在无血清神经球介质中分离出的高档胶质瘤手术标本的传播协议,为具有癌症干细胞表型的细胞进行选择。对于不能作为神经圈生长的标本,建议采取替代方案。描述了一种石蜡嵌入技术,用于维持免疫细胞化学的3D神经球结构。

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