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从胚胎前脑内皮细胞的分离和培养

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

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从胚胎前脑内皮细胞的分离和培养

DOI:

10.3791/51021-v

•

12:02 min

•

January 23, 2014

•

Peeyush Kumar T.², Anju Vasudevan²

¹Department of Psychiatry,Harvard Medical School, ²Angiogenesis and Brain Development Laboratory, Division of Basic Neuroscience,McLean Hospital

Capítulos

00:05Título
01:45Removal of Embryos and Dissection of Telencephalon
02:53Cell Isolation
04:43Magnetic Labeling and Separation
07:26Cultured Periventricular Endothelial Cell Characterization
11:24Conclusion

Summary

Tadução automática

该视频演示了一个简单而可靠的战略准备内皮细胞从内10-12天的胚胎前脑的纯培养物，并将成为研究主要集中在脑血管的许多方面非常有用。

Tags

Keywords: Embryonic Forebrain Endothelial Cells Pial Vascular Network Periventricular Vascular Network Isolation Culture Purification CD31/PECAM-1 Angiogenesis Neurovascular Development

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