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Isolering af myeloide dendritiske celler og epitelceller fra human Thymus
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1Department of General Neurology,Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology,University of Bern, 3Department of Immunology,University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery,University Clinic Tuebingen, 5Department of Neurology,University Hospital Erlangen
Capítulos
- 00:05Título
- 01:11Preparation of the Tissue
- 02:45Typical Enzymatic Tissue Digestion to Obtain a Single Cell Suspension
- 04:35Enrichment of Low Density Fraction (LDF) of Cells Using Percoll Density Separation
- 06:00Results: Representative Myeloid DC and TEC Populations Isolated from the Human Thymus
- 08:06Conclusion
Denne protokol beskriver en metode til isolering af antigen-præsenterende celler fra human thymus via forskellige trin af enzymatisk spaltning af vævet efterfulgt af densitetscentrifugering af enkelt cellesuspension og endelig magnetiske og / eller FACS-sortering af cellepopulationer af interesse.
