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膜下丘脑腹内侧神经元的电位染料成像从成年小鼠为研究葡萄糖传感

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

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膜下丘脑腹内侧神经元的电位染料成像从成年小鼠为研究葡萄糖传感

DOI:

10.3791/50861-v

•

11:10 min

•

November 27, 2013

•

Reema P. Vazirani, Xavier Fioramonti, Vanessa H. Routh

¹Department of Pharmacology and Physiology,Rutgers New Jersey Medical School, ²Center for Taste and Feeding Behavior,Universite de Bourgogne

Capítulos

00:05Título
01:18Brain Slicing and Dissection
02:35Dissociation and Culture
05:50Preparation for Membrane Potential Dye Imaging
08:23MPD Imaging
09:36Results: Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons
10:43Conclusion

Summary

Tadução automática

从成年鼠龄的单神经元的活性可以通过从特定脑区域的神经元解离，并使用荧光膜电位染料成像进行研究。通过测试响应变化的葡萄糖，这种技术可以用来研究成年腹内侧下丘脑神经元的葡萄糖敏感性。

Tags

Keywords: Membrane Potential Dye Ventromedial Hypothalamus Glucose Sensing Adult Mice Fluorescence Imaging Neuronal Activity Electrophysiology Heterogeneous Neuronal Population Energy Balance Obesity

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