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Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus
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December 19, 2013
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1Physiology Course,Marine Biological Laboratory, 2Department of Biochemistry & Biophysics,University of California San Francisco, 3Department of Physics and Astronomy,Vrije Universiteit Amsterdam, 4Max Planck Institute of Molecular Cell Biology and Genetics, 5Department of Physics,University of Illinois Urbana-Champaign
Summary
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The giant ciliate Stentor coeruleus is a classical system for studying regeneration and wound healing in single cells. By imaging Stentor cells simultaneously at low and high magnification it is possible to measure cytoplasmic flows before, during, and after wounding.
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Slabodnick, M., Prevo, B., Gross, P., Sheung, J., Marshall, W. Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus. J. Vis. Exp. (82), e50848, doi:10.3791/50848 (2013).
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