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为检验新型抗病毒药物的鉴定对蓝舌病病毒
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Assays for the Identification of Novel Antivirals against Bluetongue Virus
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为检验新型抗病毒药物的鉴定对蓝舌病病毒

DOI:
10.3791/50820-v

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12:02 min

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October 11, 2013

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, ,
1Division of Infectious Diseases, Department of Medicine,University of Alabama at Birmingham, 2Molette Laboratory for Drug Discovery, Department of Chemistry and Biochemistry,Auburn University

Capítulos

  • 00:05Título
  • 01:37Cytopathic Effect Based Assay Using the CellTiterGlo Kit
  • 03:31Dose Response Assay
  • 06:28Time-of-Addition Assay
  • 08:55Representative Antiviral Compound Assay Results
  • 11:34Conclusion

Summary

Tadução automática

Tadução automática

三个实验，包括细胞病变效应（CPE）为基础的试验中，剂量反应实验和时间的 - 加法（TOA）测定法已被开发，优化，验证和用于识别对蓝舌病毒的新的抗病毒药物（BTV），以及以确定可能的机制， - 行动（MOA）为新发现的抗病毒药物。

Tags

Antiviral AssaysBTV AntiviralsCytopathic Effect (CPE) AssayDose-response AssayMechanism Of Action (ToA) AssayAntiviral ScreeningViral LifecycleCell Culture System

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