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Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
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Neurociência
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JoVE Journal Neurociência
Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

DOI:
10.3791/50794-v

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09:42 min

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December 08, 2013

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,
1Center for Research on Occupational and Environmental Toxicology,Oregon Health & Science University, 2Department of Behavioral Neuroscience,Oregon Health & Science University

Capítulos

  • 00:05Título
  • 01:49Preparation of Hypothalamic Brain Slices
  • 04:29Patch-clamp Recording
  • 06:32Measurement of Intracellular Ca2+
  • 07:38Results: Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
  • 09:05Conclusion

Summary

Tadução automática

Tadução automática

Procedures are described to perform simultaneous recordings of membrane potential or current and changes of intracellular calcium concentration. Suprachiasmatic nucleus neurons are filled with the calcium indicator bis-fura-2 using a patch clamp electrode in the whole cell patch clamp configuration.

Tags

Keywords: Simultaneous ElectrophysiologyCalcium ImagingSuprachiasmatic NucleusCircadian ClockSynaptic TransmissionCalcium SignalingPatch ClampBrain Slice

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