Journal
/
Neurociencias
/
Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

10,746 Views

•

09:42 min

•

December 08, 2013

DOI:

10.3791/50794-v

DOI:
10.3791/50794-v

•

09:42 min
December 08, 2013

•

4 Views
,
1Center for Research on Occupational and Environmental Toxicology,Oregon Health & Science University, 2Department of Behavioral Neuroscience,Oregon Health & Science University

Summary

Automatically generated

Procedures are described to perform simultaneous recordings of membrane potential or current and changes of intracellular calcium concentration. Suprachiasmatic nucleus neurons are filled with the calcium indicator bis-fura-2 using a patch clamp electrode in the whole cell patch clamp configuration.

Explore More Videos

Simultaneous ElectrophysiologyCalcium ImagingSuprachiasmatic NucleusCircadian ClockSynaptic TransmissionCalcium SignalingPatch ClampBrain Slice

Read Article

Cite this Article

Irwin, R. P., Allen, C. N. Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons. J. Vis. Exp. (82), e50794, doi:10.3791/50794 (2013).

Download .ris file

Copy

Share Video

.

Copy