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Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
Capítulos
- 00:05Título
- 01:55Sample Preparation and Measurement of Steady-state Time Course and Active Site Titration of OGG1
- 05:53Measurement of Pre-steady-state Time Course
- 10:56Single-turnover Time Course
- 11:50Results: Kinetic Measurements of DNA Glycosylase Activity
- 14:01Conclusion
Time courses for the glycosylase activity of 8-oxoguanine DNA glycosylase are biphasic exhibiting a burst of product formation and a linear steady-state phase. Utilizing quench-flow techniques, the burst and the steady-state rates can be measured, which correspond to excision of 8-oxoguanine and release of the glycosylase from the product DNA, respectively.
