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Journal / Immunology and Infection / 培养小胶质细胞的新生和成年的中枢神经系统…
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Immunology and Infection

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JoVE Journal Immunology and Infection
培养小胶质细胞的新生和成年的中枢神经系统
 
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培养小胶质细胞的新生和成年的中枢神经系统

Article DOI: 10.3791/50647-v • 11:28 min • August 9th, 2013
August 9th, 2013 •
*1,2, *2,3, *2,3, 2
1Program in Neuroscience, Stony Brook University, 2Department of Pharmacological Sciences, Stony Brook University, 3Program in Molecular and Cellular Pharmacology, Stony Brook University
* These authors contributed equally

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我们概述方法从新生儿大脑皮层和脊髓成人的可行的小胶质细胞的高效和快速隔离/文化。解剖和电镀皮质小胶质细胞可以在90分钟内完成，与随后发生〜10天的初始剥离后的小胶质细胞的收获。

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免疫学，神经科学，神经生物学，细胞生物学，分子生物学，医学，生物医学工程，生物工程，解剖学，生理学，免疫抑制，生命科学，动物生物学，动物模型，生物化学，小胶质细胞，皮质，鼠标，新生儿，细胞培养78期，脊髓，成人，组织培养，动物模型
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