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试验样品为优化风暴超高分辨率显微镜
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Test Samples for Optimizing STORM Super-Resolution Microscopy
DOI:

16:52 min

September 06, 2013

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Capítulos

  • 00:05Título
  • 01:25Fluorescent Dextran Coating of Glass
  • 02:46Fluorescent Actin Filaments on Glass
  • 03:27Microsphere Fluorescent Beads and Fiducial Markers
  • 03:54Epidermal Growth Factor Cell Staining
  • 04:56Microscopy Acquisition of STORM Data Using Alexa 647 Dye
  • 06:28Reconstructing Super-resolution Images from Raw Data Using rainSTORM
  • 08:39Box Tracking and Drift Correction Using rainSTORM
  • 09:18Results: STORM Microscopy Assessed Using Test Samples
  • 16:26Conclusion

Summary

Tadução automática

我们描述了制备3个试样,以及如何可以被用来优化和评估STORM显微镜的性能。使用这些例子中,我们展示了如何获取原始数据,然后进行处理,以获得超分辨率图像约30​​-50 nm分辨率的细胞。

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