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Journal / Neuroscience / Optogenetic Perturbation of Neural Activity with…
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Neuroscience
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JoVE Journal Neuroscience
Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion
 

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion

Article DOI: 10.3791/50513-v • 07:07 min • July 4th, 2013
July 4th, 2013 •
1, 1, 1,2, 1
1Department of Complexity Science and Engineering, Graduate School of Frontier Sciences, The University of Tokyo, 2Department of Physics, Graduate School of Science, The University of Tokyo

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Here we describe a protocol for optogenetic manipulation of motoneuronal activity while monitoring changes in motor output (muscle contraction) in semi-intact Drosophila larvae using lasers within a conventional confocal microscope. This technique enables researchers to achieve local perturbation of neural activity in a few neuromeres to elucidate the dynamics of motor circuits.

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Optogenetic Perturbation Neural Activity Laser Illumination Semi-intact Drosophila Larvae Motion Genetic Accessibility Neuronal Components Optogenetics Spatial Resolution Ventral Nerve Cord Confocal Microscope Channelrhodopsin Halorhodopsin Neural Tissue Specific Neurons Circuit Behavior Motor Output
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