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Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
 

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae

Article DOI: 10.3791/50490-v • 09:12 min • January 17th, 2014
January 17th, 2014 •
1, 1, 1, 1
1Department of Pathology and Molecule Medicine, McMaster University

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Resumo
Automatic Translation

Colonization of the murine nasopharynx with Streptococcus pneumoniae and the subsequent extraction of adherent or recruited cells is described. This technique involves flushing the nasopharynx and collection of the fluid through the nares and is adaptable for various readouts, including differential cell quantification and analysis of mRNA expression in situ.

Tags

Inflammatory Responses Intranasal Colonization Streptococcus Pneumoniae Nasopharyngeal Colonization Invasion Lungs Bloodstream Mucosal Surface Host Defenses Lower Respiratory Tract Pneumonia Bacteraemia Mortality Rates Pneumococcal Meningitis Kinetics Immune Responses Infection Models Mouse Model Clinical Isolate Self-limiting Bacterial Colonization Carriage Events Bacterial Inoculum Intranasal Route Of Administration Resident Macrophages
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