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Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
 

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Article DOI: 10.3791/50481-v • 09:12 min • April 22nd, 2013
April 22nd, 2013 •
1, 1, 1, 1
1Department of Physics and Astronomy, University of Utah

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The system described herein employs a traditional optical trap as well as an independent holographic optical trapping line, capable of creating and manipulating multiple traps. This allows for the creation of complex geometric arrangements of refractive particles while also permitting simultaneous high-speed, high-resolution measurements of the activity of biological enzymes.

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High Resolution Microscope Optical Traps Conventional Trapping Capabilities Holographic Optical Trapping Capabilities Refractive Objects Dielectric Beads Cellular Organelles Spatial Resolution Temporal Resolution Trap Center Traditional Trap 980 Nm Wavelength Second Optical Trapping System Holographic Package Trapping Patterns Field Of View Microscope 1,064 Nm Wavelength Multiple Refractive Objects High Speed Measurements High Resolution Measurements Nanometer Scale Piconewton Scale
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