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A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
JoVE Journal
Biologia
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JoVE Journal Biologia
A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
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A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

11,722 Views

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13:22 min

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September 10, 2013

DOI:

10.3791/50439-v

DOI:
10.3791/50439-v

•

13:22 min
September 10, 2013

•

6 Views
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1Institute of Toxicology and Genetics,Karlsruhe Institute of Technology – Campus North, 2Institute of Organic Chemistry,Karlsruhe Institute of Technology – Campus North, 3Institute of Organic Chemistry,Karlsruhe Institute of Technology – Campus South

Summary

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We describe the procedure and data analysis of a chemical screening system for glucocorticoid stress hormone signaling using zebrafish larvae: the Glucocorticoid Responsive In vivo Zebrafish Luciferase activitY (GRIZLY) assay. The assay sensitively and specifically detects effects on glucocorticoid signaling by compounds that require metabolization or affect endogenous glucocorticoid production.

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Weger, B. D., Weger, M., Jung, N., Lederer, C., Bräse, S., Dickmeis, T. A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System. J. Vis. Exp. (79), e50439, doi:10.3791/50439 (2013).

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