Journal
/
Química
/
不相关的代谢组学，生物源使用超高效液相色谱 - 高分辨质谱（UPLC-HRMS）
JoVE Journal
Química
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Química
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Please note that all translations are automatically generated. Click here for the English version.

不相关的代谢组学，生物源使用超高效液相色谱 - 高分辨质谱（UPLC-HRMS）

DOI:
10.3791/50433-v

•

11:00 min

•

May 20, 2013

•

, , , ,
1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology,University of Pennsylvania

Capítulos

  • 00:05Título
  • 01:20Sample Extraction from Cells
  • 02:34Re-suspension and Filtration of Samples for UPLC
  • 03:49UPLC and Mass Spectrometer Setup
  • 06:37Differential Analysis
  • 08:47Results: Analysis of Metabolites Using UPLC-HRMS
  • 10:42Conclusion

Summary

Tadução automática

Tadução automática

不相关的代谢组学研究提供了一个假说，产生一种代谢轮廓的快照。此协议将展示从细胞，血清或组织中的代谢产物的提取和分析。代谢物甲范围进行调查，采用液 - 液固相萃取，微流超高效液相色谱/高分辨率质谱（UPLC-HRMS）耦合到差分分析软件。

Tags

Untargeted MetabolomicsUPLC-HRMSLiquid-liquid ExtractionPolar And Non-polar MetabolitesMicroflow UPLCPositive And Negative IonizationLabel-free Differential AnalysisMetabolic Pathway ScreeningBiomarker DiscoveryDrug Development

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

规模以上非靶向代谢组学剖析血清超高效液相色谱 - 质谱（UPLC-MS）

使用氢交换质谱分析蛋白质动力学

植物材料的快速高通量物种鉴定实时高分辨质谱法直接分析

液相色谱离子迁移 QTOF 质谱测定蛋白质生物中宿主细胞蛋白的 HS-MRM 法

原位水中石粒子的液体扫描电镜表征

利用二次 Nanoelectrospray 电离耦合高分辨质谱的实时呼吸分析

活蛙 (爪蟾) 胚胎单细胞代谢的微探针毛细管电泳质谱

利用毛细管区电泳串联质谱技术进行大尺度自上而下蛋白质组学

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

利用飞行时二次离子质谱法成像金属颜料界面的腐蚀

Read Article