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Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means

Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means

13,761 Views

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05:20 min

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March 02, 2013

DOI:

10.3791/50342-v

DOI:
10.3791/50342-v

•

05:20 min
March 02, 2013

•

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1Centro de Biología Celular y Molecular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), 2Acharya Nagarjuna University, 3Departamento de Medios y Creativo,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP)

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The paramagnetic properties of hemozoin are used to isolate late stages of Plasmodium falciparum-infected red blood cells growing in culture. The method is simple and fast and does not affect the subsequent invasive capabilities of the parasites.

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Plasmodium FalciparumErythrocytesMagnetic SeparationHaemozoinParamagnetismLate Stage ParasitesCulture Enrichment

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Coronado, L. M., Tayler, N. M., Correa, R., Giovani, R. M., Spadafora, C. Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means. J. Vis. Exp. (73), e50342, doi:10.3791/50342 (2013).

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