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Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means

Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means

DOI:
10.3791/50342-v

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05:20 min

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March 02, 2013

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, , , ,
1Centro de Biología Celular y Molecular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), 2Acharya Nagarjuna University, 3Departamento de Medios y Creativo,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP)

Capítulos

  • 00:01Título
  • 01:04Preparation
  • 02:21Loading
  • 02:45Washing
  • 03:16Elution
  • 03:50Loading Repetition
  • 04:44Purity Assessment
  • 05:07Applications

Summary

Tadução automática

Tadução automática

The paramagnetic properties of hemozoin are used to isolate late stages of Plasmodium falciparum-infected red blood cells growing in culture. The method is simple and fast and does not affect the subsequent invasive capabilities of the parasites.

Tags

Plasmodium FalciparumErythrocytesMagnetic SeparationHaemozoinParamagnetismLate Stage ParasitesCulture Enrichment

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