Journal
/
Biologia
/
生化和高通量的微观评估的脂肪含量<em>秀丽隐杆线虫</em
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
Please note that all translations are automatically generated. Click here for the English version.

生化和高通量的微观评估的脂肪含量<em>秀丽隐杆线虫</em

DOI:
10.3791/50180-v

•

16:07 min

•

March 30, 2013

•

, , ,
1Center for Human Genetic Research and Department of Medicine,Massachusetts General Hospital and Harvard Medical School, 2Department of Earth, Atmospheric, and Planetary Sciences,Massachusetts Institute of Technology

Capítulos

  • 00:05Título
  • 02:06Stamp Frozen Library Plates onto Rectangular Amp/Tet LB Agar Plates
  • 03:16Grow and Seed RNAi Bacterial Clones and Add Worms to RNAi Plates
  • 05:49Fix and Stain Worms in Nile Red and Image on a High-throughput Scope
  • 07:22Wash Worms and Image on a High-throughput Microscope
  • 08:31Biochemical Lipid Determination Using Solid Phase Extraction and Gas Chromatography-mass Spectrometry
  • 12:02Representative Fat Mass Content Results
  • 15:39Conclusion

Summary

Tadução automática

Tadução automática

我们提出了强大的生化和微观的研究方法<em>秀丽隐杆线虫</em>脂质店。一种快速，简单，固定，染色过程利用荧光脂滴成像光谱特性的亲脂性染料尼罗红。然后，我们提出的甘油三酯和磷脂固相萃取和气相色谱 - 质谱法的生化测定。

Tags

Keywords: Caenorhabditis ElegansLipid DropletsNile Red StainingHigh-throughput MicroscopyTriglyceridesPhospholipidsGas Chromatography-mass Spectrometry

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/50180?article_type=v

分离和<em在体外激活线虫精子
check_url/pt/50180?article_type=v

实心板为基础的饮食限制在线虫</em
check_url/pt/50180?article_type=v

高通量筛选和生物传感与荧光 C。线虫菌株
check_url/pt/50180?article_type=v

时间推移显微镜早期胚胎发育线虫
check_url/pt/50180?article_type=v

定量和自动化高通量的全基因组RNAi屏幕 C。线虫</em
check_url/pt/50180?article_type=v

基本的<em>线虫</em>方法：同步和观察
check_url/pt/50180?article_type=v

前列腺素的提取与分析<em>线虫</em
check_url/pt/50180?article_type=v

高通量筛选转录后调控基因的化学调节剂
check_url/pt/50180?article_type=v

栽培<em>秀丽隐杆线虫</em>在三个维度实验室
check_url/pt/50180?article_type=v

线虫的耗氧率测定

Read Article