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Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo
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Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo
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May 29, 2013
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How neuronal networks are established in the embryonic brain is a fundamental question in developmental neurobiology. Here we combined an electroporation technique with novel genetic tools, such as Cre/Lox–plasmids and PiggyBac-mediated DNA transposition system in the avian hindbrain to label dorsal interneurons and track their axonal projections and synaptic targets at various developmental stages.
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Kohl, A., Hadas, Y., Klar, A., Sela-Donenfeld, D. Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo. J. Vis. Exp. (75), e50136, doi:10.3791/50136 (2013).
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