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实验方法测试的影响，在怀孕期间对酒精诱导的细胞凋亡在C57BL / 6小鼠神经营养肽，ADNF-9

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Neurociência

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JoVE Journal Neurociência

Experimental Methods for Testing the Effects of Neurotrophic Peptide, ADNF-9, Against Alcohol-induced Apoptosis during Pregnancy in C57BL/6 Mice

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实验方法测试的影响，在怀孕期间对酒精诱导的细胞凋亡在C57BL / 6小鼠神经营养肽，ADNF-9

DOI:

10.3791/50092-v

•

09:50 min

•

April 24, 2013

•

Youssef Sari

¹Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences,University of Toledo

Capítulos

00:05Título
01:26Alcohol Dosing and Trophic Peptide Treatment
02:42Dissection of Fetuses and Microdissection of Fetal Brains
04:32Embedding Fetal Brains in Gelatin for Sectioning
06:07TUNEL Reaction for Detection of Apoptosis
08:16Results: Effects of the Neurotrophic Peptide, ADNF-9 Against Alcohol-induced Apoptosis
09:27Conclusion

Summary

Tadução automática

这里重点提出的实验设计，研究酒精暴露在怀孕期间胎儿大脑细胞凋亡和应用营养神经肽的影响。从养殖到胎儿大脑的集合的详细描述。细胞凋亡的测定技术进行详细说明。

Tags

Keywords: Experimental Methods Neurotrophic Peptide ADNF-9 Alcohol-induced Apoptosis Pregnancy C57BL/6 Mice Fetal Brain Growth Microdissection TUNEL Assay Apoptosis Cell Death Fetal Alcohol Exposure Liquid Diet Breeding Vibratome Sectioning

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