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Sekventiell fotoblekningsreaktion att avgränsa Single Schwann celler vid den neuromuskulära förbindelsen
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1Lehrstuhl für Biomolekulare Sensoren,Technische Universität München, 2Center for Integrated Protein Science (Munich) at the Institute of Neuroscience,Technische Universität München, 3TUM Institute for Advanced Study and German Center for Neurodegenerative Diseases,Technische Universität München, 4Munich Cluster for Systems Neurology (SyNergy),Technische Universität München
Capítulos
- 00:05Título
- 01:36Preparing the Triangularis Sterni Explant
- 04:34Photobleaching and Time-lapse Microscopy
- 07:52Fixing and Dissecting the Explant for Immunohistochemistry
- 09:45Triangularis Sterni Visualization with GFP in Schwann Cells
- 10:42Conclusion
Visualisera enskilda celler i tätt packade vävnader såsom terminal Schwann celler (SCS) vid neuromuskulär korsningar (NMJs), är en utmaning. "Sekventiell fotoblekningsreaktion" tillåter avgränsar enda terminal SCS, till exempel i triangularis sterni muskel explantation, en bekväm nerv-muskel preparat, där sekventiell blekning kan kombineras med time-lapse avbildning och<em> Post-hoc</em> Immunostainings.
Tags
Sequential Photo-bleachingSchwann CellsNeuromuscular JunctionMotor NeuronsTransgenic MiceSynaptic StructureSynaptic FunctionMyelinating Axonal SCsNodes Of RanvierSaltatory Action Potential PropagationGlial CellsNeurotransmissionDebris DigestionAxon RegenerationNon-myelinating Terminal SCsLight MicroscopyDye FillingTime-lapse RecordingsMulti-spectral Labeling
