/
/
Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction
10,474 Views
•
11:12 min
•
January 11, 2013
DOI:
1Lehrstuhl für Biomolekulare Sensoren,Technische Universität München, 2Center for Integrated Protein Science (Munich) at the Institute of Neuroscience,Technische Universität München, 3TUM Institute for Advanced Study and German Center for Neurodegenerative Diseases,Technische Universität München, 4Munich Cluster for Systems Neurology (SyNergy),Technische Universität München
Summary
Automatically generated
Visualizing individual cells in densely packed tissues, such as terminal Schwann cells (SCs) at neuromuscular junctions (NMJs), is challenging. "Sequential photo-bleaching" allows delineating single terminal SCs, for instance in the triangularis sterni muscle explant, a convenient nerve-muscle preparation, where sequential bleaching can be combined with time-lapse imaging and post-hoc immunostainings.
Explore More Videos
Sequential Photo-bleachingSchwann CellsNeuromuscular JunctionMotor NeuronsTransgenic MiceSynaptic StructureSynaptic FunctionMyelinating Axonal SCsNodes Of RanvierSaltatory Action Potential PropagationGlial CellsNeurotransmissionDebris DigestionAxon RegenerationNon-myelinating Terminal SCsLight MicroscopyDye FillingTime-lapse RecordingsMulti-spectral Labeling
Read Article
Cite this Article
Brill, M. S., Marinković, P., Misgeld, T. Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction. J. Vis. Exp. (71), e4460, doi:10.3791/4460 (2013).
Copy