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Sekventiel fotoblegende at afgrænse Single Schwann-celler ved den neuromuskulære overgang
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1Lehrstuhl für Biomolekulare Sensoren,Technische Universität München, 2Center for Integrated Protein Science (Munich) at the Institute of Neuroscience,Technische Universität München, 3TUM Institute for Advanced Study and German Center for Neurodegenerative Diseases,Technische Universität München, 4Munich Cluster for Systems Neurology (SyNergy),Technische Universität München
Capítulos
- 00:05Título
- 01:36Preparing the Triangularis Sterni Explant
- 04:34Photobleaching and Time-lapse Microscopy
- 07:52Fixing and Dissecting the Explant for Immunohistochemistry
- 09:45Triangularis Sterni Visualization with GFP in Schwann Cells
- 10:42Conclusion
Visualisering individuelle celler i tætpakkede væv, såsom terminale Schwann-celler (SCS) i neuromuskulære junctions (NMJs), er udfordrende. "Sekventiel fotoblegende" tillader afgrænse enkelt terminal SCS, for eksempel i triangularis sterni muskel eksplantation, en bekvem nerve-muskel forberedelse, hvor sekventiel blegning kan kombineres med time-lapse imaging og<em> Post-hoc</em> Immunostainings.
Tags
Sequential Photo-bleachingSchwann CellsNeuromuscular JunctionMotor NeuronsTransgenic MiceSynaptic StructureSynaptic FunctionMyelinating Axonal SCsNodes Of RanvierSaltatory Action Potential PropagationGlial CellsNeurotransmissionDebris DigestionAxon RegenerationNon-myelinating Terminal SCsLight MicroscopyDye FillingTime-lapse RecordingsMulti-spectral Labeling
