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连续光漂白划定单雪旺细胞在神经肌肉接头处
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Neurociência
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JoVE Journal Neurociência
Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction
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连续光漂白划定单雪旺细胞在神经肌肉接头处

DOI:
10.3791/4460-v

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11:12 min

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January 11, 2013

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, ,
1Lehrstuhl für Biomolekulare Sensoren,Technische Universität München, 2Center for Integrated Protein Science (Munich) at the Institute of Neuroscience,Technische Universität München, 3TUM Institute for Advanced Study and German Center for Neurodegenerative Diseases,Technische Universität München, 4Munich Cluster for Systems Neurology (SyNergy),Technische Universität München

Capítulos

  • 00:05Título
  • 01:36Preparing the Triangularis Sterni Explant
  • 04:34Photobleaching and Time-lapse Microscopy
  • 07:52Fixing and Dissecting the Explant for Immunohistochemistry
  • 09:45Triangularis Sterni Visualization with GFP in Schwann Cells
  • 10:42Conclusion

Summary

Tadução automática

Tadução automática

可视化的单个细胞密集的组织，如终端雪旺氏细胞（SC）的神经肌肉接头（NMJs），是具有挑战性的。 “连续光漂白”可以划定单一的终端南海，例如在的三角sterni肌肉外植体，方便神经肌肉准备，连续的漂白可以结合时间推移成像<em>事后</em> immunostainings。

Tags

Sequential Photo-bleachingSchwann CellsNeuromuscular JunctionMotor NeuronsTransgenic MiceSynaptic StructureSynaptic FunctionMyelinating Axonal SCsNodes Of RanvierSaltatory Action Potential PropagationGlial CellsNeurotransmissionDebris DigestionAxon RegenerationNon-myelinating Terminal SCsLight MicroscopyDye FillingTime-lapse RecordingsMulti-spectral Labeling

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