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Imunologia e Infecção

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肽：MHC四聚体为基础的抗原表位特异性的T细胞的富集

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

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肽：MHC四聚体为基础的抗原表位特异性的T细胞的富集

17,601 Views

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13:58 min

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October 22, 2012

DOI:

DOI:

•

13:58 min

October 22, 2012

•

7 Views

Francois P. Legoux¹, James J. Moon¹

¹Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit,Massachusetts General Hospital and Harvard Medical School

Summary

此协议描述了使用的肽：MHC四聚体和磁性微珠分离低频表位特异性的T细胞群体，并通过流式细胞术分析它们。这种方法能够直接研究内源性T细胞群的兴趣<em在体内</em实验系统。

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