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Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
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Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
Capítulos
- 00:05Título
- 01:26Dissection
- 06:41Dissociation of Tissue and Plating of Cells
- 08:24Results: Analysis of Xenopus laevis Embryonic Retinal Tissue
- 09:37Conclusion
Xenopus laevis provides an ideal model system for studying cell fate specification and physiological function of individual retinal cells in primary cell culture. Here we present a technique for dissecting retinal tissues and generating primary cell cultures that are imaged for calcium activity and analyzed by in situ hybridization.
Tags
DissectionCultureAnalysisXenopus LaevisNeural PlateVertebrate RetinaClinical ResearchBasic ResearchRetinal DiseaseNeuronal Cell Type DeterminationDifferentiationGanglion CellsAmacrine CellsHorizontal CellsPhotoreceptorsBipolar CellsMüller Glial CellsLayered StructurePrimary Cell CulturePresumptive Retinal CellsDevelopmental StagesCell Fate Specification
