/
/
Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
13,710 Views
•
10:22 min
•
December 23, 2012
DOI:
Summary
Automatically generated
Xenopus laevis provides an ideal model system for studying cell fate specification and physiological function of individual retinal cells in primary cell culture. Here we present a technique for dissecting retinal tissues and generating primary cell cultures that are imaged for calcium activity and analyzed by in situ hybridization.
Explore More Videos
DissectionCultureAnalysisXenopus LaevisNeural PlateVertebrate RetinaClinical ResearchBasic ResearchRetinal DiseaseNeuronal Cell Type DeterminationDifferentiationGanglion CellsAmacrine CellsHorizontal CellsPhotoreceptorsBipolar CellsMüller Glial CellsLayered StructurePrimary Cell CulturePresumptive Retinal CellsDevelopmental StagesCell Fate Specification
Read Article
Cite this Article
McDonough, M. J., Allen, C. E., Ng-Sui-Hing, N. A., Rabe, B. A., Lewis, B. B., Saha, M. S. Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue. J. Vis. Exp. (70), e4377, doi:10.3791/4377 (2012).
Copy