É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Determination of the Gas-phase Acidities of Oligopeptides
10,928 Views
•
11:00 min
•
June 24, 2013
DOI:
Summary
Automatically generated
The determination of the gas-phase acidities of cysteine-containing oligopeptides is described. The experiments are performed using a triple quadrupole mass spectrometer. The relative acidities of the peptides are measured using collision-induced dissociation experiments, and the quantitative acidities are determined using the extended Cooks kinetic method.
Explore More Videos
Gas-phase AciditiesOligopeptidesAmino Acid ResiduesFolded ProteinsCysteine ResidueN-terminusC-terminusExperimental StudiesCondensed PhaseAqueous SolutionsSolvent EffectsActive SitesInterior RegionIntrinsic Acid-base PropertiesSolvent-free EnvironmentMeasurement MethodCooks Kinetic MethodMass SpectrometerElectrospray Ionization (ESI) Ion Source
Read Article
Cite this Article
Ren, J., Sawhney, A., Tian, Y., Padda, B., Batoon, P. Determination of the Gas-phase Acidities of Oligopeptides. J. Vis. Exp. (76), e4348, doi:10.3791/4348 (2013).
Copy